The National Information and Documentation Centre (NIDOC) affiliated to the Academy of Scientific Research and Technology (ASRT)Novel Research in Microbiology Journal2537-02864620201201Corona virus disease (COVID-19): Epidemiology, Pathogenesis, Diagnosis, Therapeutics under trial and Prevention1015102822781110.21608/nrmj.2020.130848ENBijayKumar ShresthaDepartment of Microbiology, Central Campus of Technology, Tribhuvan University, Hattisar, Dharan, Nepal0000-0002-6542-829XJenishShakyaDepartment of Microbiology, Central Campus of Technology, Tribhuvan University, Hattisar, Dharan, NepalManitaTumbahangpheDepartment of Microbiology, Central Campus of Technology, Tribhuvan University, Hattisar, Dharan, NepalBidhyaDhunganaDepartment of Microbiology, Central Campus of Technology, Tribhuvan University, Hattisar, Dharan, NepalRomikaShresthaDepartment of Microbiology, Central Campus of Technology, Tribhuvan University, Hattisar, Dharan, NepalJyotiLimbuDepartment of Microbiology, Central Campus of Technology, Tribhuvan University, Hattisar, Dharan, NepalJournal Article20201002The unforeseeable outbreak of novel coronavirus called Severe Acute Respiratory Disease Corona Virus-2 (SARS-CoV-2) from Wuhan city of China has become pandemic, associated with great mortality and morbidity across the globe. The virus is transmitted through air droplets from infected person to healthy person. Beta-coronavirus SARS-CoV-2 that shares common relativeness with Severe acute respiratory syndrome (SARS) and Middle East Respiratory syndrome (MERS) affects the lungs, which is manifested as pneumonia clinically characterized by; fever, dry cough, shortness of breath and fatigue. Real time-Polymerase chain reaction (RT-PCR) and Radiological methods such as Computerized Tomography of chest (CT-scan) are the most preferred diagnostic tools. In fact, the CT-scan of chest is considered to be most sensitive, accurate and a rapid diagnostic tool to remove false negative results, and hence stands to be an efficient diagnostic tool for confirming Corona Virus Disease-19 (COVID-19) infection. Therefore, RT-PCR along with CT-scan reports help to correctly confirm COVID-19, which in turn are effective in diagnosis, treatment and management of COVID-19 patients. This review aimed to understand the epidemiology, pathogenesis, diagnosis, therapeutics and preventive measures of COVID-19.https://nrmj.journals.ekb.eg/article_227811_ff9b8d29c2cc8420971a17299339783e.pdfThe National Information and Documentation Centre (NIDOC) affiliated to the Academy of Scientific Research and Technology (ASRT)Novel Research in Microbiology Journal2537-02864620201201Diversity of aquatic Pythium and Phytopythium spp. from rivers and a pond of Gifu city, Japan1029104422781210.21608/nrmj.2020.130851ENHaniM. A. AbdelzaherBiology Department, College of Science, Jouf University, P.O. Box: 2014, Sakaka, Saudi ArabiaKojiKageyamaLaboratory of Molecular Biology, River Basin Research Center, Gifu University, JapanJournal Article20201117Pythiaceous fungi have variety of occurrences in different environments. If these fungi are present in water intended to irrigate crops, they pose high risk as pathogens. <em>Pythium adhaerens</em>, <em>P. aquatile</em>, <em>P. diclinum</em>, <em>P. dissotocum</em>, <em>P. pachycaule</em> and <em>P. torulosum</em>, in addition to asexual isolates of <em>P. dissotocum</em> (<em>Pythium</em> “group F”) and <em>Phytopythium helicoides</em> (the former, <em>Pythium</em> “group P”) were isolated from 5 rivers and a pond in Gifu, Japan, and their diversity were studied. All of the isolated species have been previously recorded from aquatic habitats except for <em>P. pachycaule</em>. Sequencing of the internal transcribed spacer regions of ribosomal DNA (rDNA-ITS) including the 5.8SrDNA of these fungi confirmed primary identification based on morphological characteristics. This study proves the dense presence of different species of these Pythiaceous fungi, based on the latest modern identification methods, through which a new genus of <em>Pythium</em> was recorded and divided into two species. The aim of this study was to study the biological diversity of <em>Pythium</em>, which is pathologically important for many crop plants that are irrigated with water from these five rivers and the pond in Gifu, Japan. https://nrmj.journals.ekb.eg/article_227812_c0cff3a744162a7f2a74ecd4fa019952.pdfThe National Information and Documentation Centre (NIDOC) affiliated to the Academy of Scientific Research and Technology (ASRT)Novel Research in Microbiology Journal2537-02864620201201Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases1045105622781310.21608/nrmj.2020.130852ENFatmaM. Abdel BasetBotany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptNouraSh. A. HagaggiBotany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptFrancisF. HezayenBotany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptUsamaM. Abdul- RaoufBotany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptJournal Article20201120<em>Calotropis procera</em> (Aiton) W.T. Aiton is a shrub belongs to family Asclepiadaceae which known by its medicinal properties. It is a widely growing plant distributed in tropical and sub-tropical Africa, and America. This study is the first report which highlights the diversity of bacterial endophytes from <em>C. procera</em> as sources of numerous hydrolytic exo-enzymes. Endophytic bacteria were isolated from all plant parts such as; roots, stems, leaves, flowers, fruits and latex. <em>Bacillus</em> was the prevalent genus. At the species level, the bacterial diversity was high. Eight representative species were isolated including; <em>Citricoccus alkalitolerans</em> (Cps2) (NR025771), <em>Bacillus cereus</em> (Cps1) (NR074540), <em>B. pumilus</em> (Cps3) (NR112637), <em>B. firmus</em> (Cpl1) (NR025842), <em>B. niabensis</em> (Cpl3) (NR043334), <em>B. subtilis</em> (Cpl4) (NR113265), <em>B. amyloliquefaciens</em> (Cpl10) (NR041455) and <em>B. subtilis</em> subsp. <em>spizizenii</em> (Cpl13) (NR112686). Results of the current study emphasized that <em>C. procera</em> plant hosts diverse endophytic bacteria, which are potential producers of several economically important hydrolytic enzymes i.e., amylase, protease, cellulase, lipase and L-asparaginase. The aims of the current study were to identify the endophytic bacteria associated with the different organs of the medicinal plant <em>C. procera</em>, and to evaluate their potentialities to produce diverse extracellular hydrolytic enzymes.https://nrmj.journals.ekb.eg/article_227813_4b4f93f0ac876ac27c99dbb028d8f91e.pdf