Pant, M., Shrestha, D., Thapa, S. (2018). Antibiogram of bacterial species causing skin wound infections. Novel Research in Microbiology Journal, 2(3), 53-60. doi: 10.21608/NRMJ.2018.8153
Mahesh Pant; Dipti Shrestha; Shovana Thapa. "Antibiogram of bacterial species causing skin wound infections". Novel Research in Microbiology Journal, 2, 3, 2018, 53-60. doi: 10.21608/NRMJ.2018.8153
Pant, M., Shrestha, D., Thapa, S. (2018). 'Antibiogram of bacterial species causing skin wound infections', Novel Research in Microbiology Journal, 2(3), pp. 53-60. doi: 10.21608/NRMJ.2018.8153
Pant, M., Shrestha, D., Thapa, S. Antibiogram of bacterial species causing skin wound infections. Novel Research in Microbiology Journal, 2018; 2(3): 53-60. doi: 10.21608/NRMJ.2018.8153
Antibiogram of bacterial species causing skin wound infections
1Department of Microbiology Kathmandu College of Science and Technology, Kamalpokhari, Kathmandu, Nepal
2Department of Pathology Institute of Medicine (TUTH) and IFCH, Maharajgung, Kathmandu, Nepal
Receive Date: 11 May 2018,
Revise Date: 04 June 2018,
Accept Date: 19 June 2018
Abstract
Wounds occur when the integrity of any tissues is compromised. Infection causes significant increase in costs, morbidity, and potential mortality. This study was conducted during the period from July, 2015 to January, 2016 with the aims of identifying the etiological agents causing skin wound infections, and their antibiotic susceptibility profile among patients visiting International Friendship Children’s Hospital (IFCH), Maharajgunj, Kathmandu, Nepal. Specimens were processed by conventional culture technique and antibiogram of isolates were done by modified Kirby-Bauer disc-diffusion method. Out of 219 skin pus samples, 132 (60.3%) were reported to be bacterial culture positive. Eight different bacterial species were identified as; S. aureus 75 (56.8%), Coagulase negative S. aureus (CONS) 20 (15.2%), Escherichia coli 13 (9.8%), Citrobacter spp. 7 (5.3%), Pseudomonasaeruginosa 5 (3.7%), Klebsiella spp. 5 (3.7%), Proteus spp. 5 (3.7%) and Enterobacter spp. 2 (1.5%), all were isolated from culture positive specimens. Antibiotic susceptibility test (AST) of all Gram-negative isolates showed that Colistin and Imepenum were the most effective antibacterial drugs. Out of total 75 S. aureus isolates, all were reported to be susceptible to Vancomycin, whereas, 23 (30.7%) were resistant to methicillin. This study reported that S. aureus strains were the predominant isolates. Prevalence of multi-drug resistant strains of S. aureus is increasing. Current results demonstrated that antibiotic resistance in Gram-positive and Gram-negative bacteria is increasing in alarming trends that lead to failure of treatment.
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