Document Type : Original Article
Authors
1
Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, Dokki, 12622, Giza, Egypt
2
Biochemistry Department, Cancer biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, 12622, Giza, Egypt
3
Department of Pretreatment and Finishing of Cellulosic based Textiles, Institute of Textile Research and Technology, National Research Centre, 33 EL Buhouth St., Dokki, Giza 12622, Egypt
Abstract
Bimetallic nanoparticles (BNPs) have garnered a great interest rather than monometallic nanoparticles (NPs) in terms of biotechnological applications due to their enhanced properties. They have a growing interest as promising biomedical agents for drug delivery, antibacterial, and anticancer treatments due to their significant permeability and retention effects. Both of nano-size copper (Cu) and selenium (Se) exhibited a significant activity in biological treatment and drug delivery systems. Consequently, the present work aimed to focus on the novel biogenic synthesis of Cu-Se BNPs using cell-free extract of a marine bacterium, and studying their anticancer and antioxidant activities. Based on 16S rDNA sequencing, the isolated marine bacterium was identified Bacillus amyloliquefaciens by phylogenetic analysis. Biogenic synthesis of Cu-Se BNPs was optimized by studying the effect of some physiological factors such as reaction time, pH, and temperature. Based on the reaction conditions, the biologically synthesized Cu-Se NPs was obtained with different particle size that ranged from 15.7-106.0 nm. The spherical small sized (15.7 nm) bimetallic Cu-Se BNPs displayed anticancer activity against HepG2 and MDA cell lines, while the cell viability was reduced by 87 % and 81 %, respectively. The estimated IC50 values were 696.4 µg/ ml for HepG2 and 273.9 µg/ ml for MDA, while scavenging capacity (SC) of Cu-Se BNPs (SC50 = 305.3 µM) showed lower ability to 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) scavenging compared to ascorbic acid (SC50 = 95.9 µM).
Keywords
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